Coding

Part:BBa_K613020:Design

Designed by: Nadine Guenat   Group: iGEM11_EPF-Lausanne   (2011-09-20)

TetR P39Q Y42M L197S mutant


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The mutation was introduced by PCR-induced mutagenesis, based on data from [http://www.sciencedirect.com/science/article/pii/S0022283697915400 Helbl and Hillen, 1998].

Source

The TetR gene was PCR-amplified from the Repressilator plasmid. We then introduced the V36F mutation by site-directed mutagenesis. Parts of this mutation had already been described by Helbl and Hillen (1998).

References